DNA Quantitation in Next-Generation Sequencing Library Workflows

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The QuantiFluor® dsDNA System was developed using the fluorescence module of the GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths, including the Quantus™ Fluorometer. Each QuantiFluor® dsDNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl. QuantiFluor® dsDNA System Features & Benefits:   Specificity - Highly specific to dsDNA, minimal binding to ssDNA, RNA, protein and interfering compounds. Sensitivity - Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye and can detect as little as 50pg/ml. Ease of Use - System includes all required reagents to quickly set up and quantitate dsDNA. Instrument Compatibility - Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.

  The Quantus™ Fluorometer is an easy-to-use and affordable single-tube fluorometer.   The highly sensitive instrument is preprogrammed with QuantiFluor® Dye protocols for single-stranded DNA, double-stranded DNA and RNA quantitation but is also compatible with other fluorescent assays. Quantus™ Fluorometer does the math for you and displays your concentration after each measurement automatically. No computer is required. The Quantus™ Fluorometer will save and display the 20 most recent measurements. However, the fluorometer can be connected to a computer to transfer data in real time. Quantus™ Fluorometer Features: High Performance - Integrated with QuantiFluor® Dyes for high sensitivity, broad dynamic range and target specificity. Great for low-level sample quantitation such as FFPE or viral samples. Increased Sensitivity - Significantly increased sensitivity over absorbance at 260nm (NanoDrop®) for those samples that are low in concentration. Ten times more sensitive than Qubit® 2.0. A detection limit of 50pg/ml, compared to 500pg/ml for the Qubit® 2.0. With a customized low standard curve, the detection limit can read as low as 1pg/ml. Easy-to-Use Workflow and Navigation - Flexible with custom protocols and user-defined settings. PC software for data management workflow. Affordable Price - Cost-effective to easily incorporate into your laboratory.