PathDetect Egr-1 Cis-Reporting System
Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems. When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element.
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Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems. When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element.
For ultimate flexibility, our pLuc-MCS expression vector includes a multiple cloning site to facilitate insertion of your enhancer or transcription element. The system also includes positive and negative controls that ensure the success of your experiments.
- Identify pathway-specific transcription activation
- Easier and faster than blotting or gel-shift assays
- Sensitive, quantitative assays
For Research Use Only. Not for use in diagnostic procedures
