Odyssey DLx Infrared Imaging System

Infrared fluorescence detection with Odyssey Family Imaging Systems provides a quantitative two-color detection method for Western blots. This application note is designed to help you achieve success with NIR Western blot detection methods.

Peptides labeled with fluorescent dyes are important as probes for in vivo imaging and as substrates for enzyme activity assays. Near-infrared (NIR) fluorophores such as IRDye® 800CW (excitation 780 nm; emission 794 nm) can offer improved sensitivity because of low NIR autofluorescence from tissues, cells, biological materials, or drug compounds. Fluorophores can also be combined with appropriate quencher dyes to create fluorogenic peptide probes, such as those used for protease activity detection. This application note provides an overview of IRDye® Peptide Labeling with LI-COR’s Odyssey® Classic, Odyssey CLx, Odyssey Sa, and Aerius Imaging Systems.

This article presents several useful factors to consider when imaging mice using the Odyssey CLx infrared imaging systems.

This document describes a method to determine optimal blocking conditions for NIR Western blot detection with the Odyssey family of Imagers. The specific lysate and antibodies used in your system will be evaluated in four different blocking buffer systems. The buffering system will also be evaluated in this experiment.

In this note find tips to make the most of your near-infrared (NIR) Western Blot. Some of the factors, including antibody quality, blocking agent selection, contamination and membrane variability that may alter the performance and imaging of a NIR Western blot are discussed. Methods of maximizing performance using the Odyssey Infrared Imaging System and Odyssey software are also described.

This application note outlines IRDye infrared dyes, a useful tool for fluorescent labelling of protein and cellular assays, biochemical assays,microscopy, and in vivo molecular imaging. The dyes are bright, have excellent water solubility,and exhibit low non-specific binding. Background fluorescence is reduced at NIR wavelengths, enabling a variety of fluorescent applications that were previously impractical.

This application note introduces the quencher dye, IRDye QC-1 for use with near-infrared (NIR) applications. IRDye QC-1 is an excellent quencher with essentially no inherent fluorescence and good water solubility, for use in many biological applications. This quencher dye also has the widest available quenching range, with the ability to quench both visible and near infrared fluorophores (~500 nm to ~900 nm).

The Odyssey Infrared Imaging System provides a sensitive and flexible choice for imaging two-dimensional gel electrophoresis (2DE) gels and blots. Here, LI-COR presents an overview of the 2DE process and viable options for Odyssey detection of proteins separated by 2DE.

Diet considerations in 'in vivo' animal imaging are investigated in this application note from LI-COR. Near-infrared (NIR) optical imaging is a useful tool for in vivo study of animal samples, and diet prior to NIR has been shown to affect the quality of the result. A variety of diets are discussed with some recommendations as to various diets to avoid for optimum in vivo imaging outcome.

The Odyssey® Infrared Imaging system allows you to detect target proteins while still embedded in the gel using near-infrared secondary antibodies. Using near-infrared fluorescence detection methods for In-Gel Westerns makes this a powerful technique. Here, a protocol for In Gel Western analysis and tips for its optimisation is presented.

In this video, LI-COR explains how its Odyssey imaging systems promise to provide excellent data quality including three colour western blots, in-cell western assays, and cell analysis.