HybridSPE-PLus Plates Pack of One
The Next Generation in Hybridspe-Phospholipid Technology
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The Next Generation in Hybridspe-Phospholipid Technology
The new HybridSPE-PLus plates offer the same time-proven chemistry of the traditional HybridSPE-Phospholipid plates, coupled with plate manufacturing enhancements that stem from years of production experience. These manufacturing enhancements have increased the well to well flow consistency and reduced the sample hold up volumes. Overall this improves analyte recoveries and assay reproducibility. A further description is found on this video from Sigma Aldrich, or See more on their website. You can also read about the HybridSPE-Plus being applied to measure Digoxin and Digitoxin in plasma in an application note here.
Features:
- Simple 2-3 step standard methodology analogous to traditional protein precipitation provides simultaneous removal of both proteins and phospholipids
- Unique Lewis acid/base retention mechanism effectively removes phospholipids from both hydrophilic and hydrophobic analytes of interest (unlike competitive chemistries) eliminating phospholipid-induced ion suppression
- Highly reproducible, consistent product performance
- Provides cleaner samples for longer column life and less instrument downtime
- Available in 96-well and 1 mL cartridge dimensions
Overcoming the Matrix Effect in LC/MS of Serum or Plasma Samples Using Two Distinct Sample Prep Approaches
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it. Advancements in electrospray ionization mass spectrometry (ESI-MS) have changed the way identification and quantification of small molecules in biological fluids is conducted. Systems are capable of routinely detecting sub-femtogram levels of analytes. This report demonstrates that targeted phospholipid depletion using HybridSPE-Phospholipid and analyte enrichment using biocompatible SPME, both provide effective means to reduce matrix interference that can rob the method of sensitivity, reproducibility, precision, and accuracy.
Rapid, Sensitive, and Quantitative LC/MS/MS Determination of Digitoxin and Digoxin in Plasma
This application note describes the development of an LC/MS/MS method to measure digoxin and Digitoxin levels in the body, as these glycosides are used for treating heart conditions. Current methods using immunoassays are not selective between the two compounds due to the similarities between the molecules.
