Overcoming the Matrix Effect in LC/MS of Serum or Plasma Samples Using Two Distinct Sample Prep Approaches

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it. Advancements in electrospray ionization mass spectrometry (ESI-MS) have changed the way identification and quantification of small molecules in biological fluids is conducted. Systems are capable of routinely detecting sub-femtogram levels of analytes. This report demonstrates that targeted phospholipid depletion using HybridSPE-Phospholipid and analyte enrichment using biocompatible SPME, both provide effective means to reduce matrix interference that can rob the method of sensitivity, reproducibility, precision, and accuracy.