Products & ReviewLife Sciences

High Sensitivity DNA ScreenTape Analysis

Agilent Technologies5067-5593Available: Worldwide

High Sensitivity D5000 Reagents. For the high sensitivity analysis of DNA from 100 to 5000 bp. Includes ladder and sample buffer sufficient for 105 samples Order with 5067-5592.

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Average Rating 4.0

|2Scientists have reviewed this product

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Does what it promises, easy to use

 

Average Rating 4.0

Application Area:

Fragment Analysis for NGS Libraries

An easy to use product, high accuracy

Review Date: 16 May 2023 | Agilent Technologies

It serves as a good assessment before the more expensive sequencing experiments!

 

Average Rating 4.0

Application Area:

TapeStation analysis

The results are reproducible and we use it a lot in the core facility for assessing of quality for RNAseq.

Review Date: 9 Dec 2021 | Agilent Technologies

High Sensitivity D5000 Reagents. For the high sensitivity analysis of DNA from 100 to 5000 bp. Includes ladder and sample buffer sufficient for 105 samples Order with 5067-5592. For Research Use Only. Not for use in diagnostic procedures.

Application NoteLife Sciences

Performance characteristics of the RNA and the high sensitivity RNA ScreenTape assays for the 4150 TapeStation System

The Agilent 4150 TapeStation system is an automated electrophoresis solution for fast and reliable nucleic acid sample quality control. The entire Agilent RNA and DNA ScreenTape portfolio is applicable on the 4150 TapeStation system. The Agilent RNA ScreenTape assays enable separation, integrity, and quantity analysis of total RNA samples from both eukaryotic and prokaryotic origin. The RNA integrity number equivalent (RINe) provides objective assessment of RNA degradation. The 4150 TapeStation system together with the RNA ScreenTape assays is perfectly suited for RNA quality control at low throughput need. In this technical overview, Agilent Technologies focuses on the performance characteristics of the RNA ScreenTape assay and the Agilent High Sensitivity RNA ScreenTape assay (HS RNA ScreenTape assay) on the 4150 TapeStation system regarding RNA integrity analysis, sensitivity, and quantification. The performance of the 4150 TapeStation system was compared to the 4200 TapeStation system using the same assays to demonstrate full compatibility. Furthermore, the Agilent 2100 Bioanalyzer system, together with the corresponding RNA assays as widely accepted standards for RNA quality control, were applied for a benchmarking of all three systems.


Application NoteLife Sciences

The European Reference Genome Atlas initiative: A collaborative effort to advance our understanding of European biodiversity

Reference genomes are a powerful method to gain complete insight into the biology of unique species within our communities. They are critical for understanding the function of biodiversity in protecting threatened species, a concern of scientists worldwide. Considering this, researchers from across Europe decided to collaborate and form the European Reference Genome Atlas (ERGA) initiative. ERGA is a pan-European consortium of multidisciplinary scientists dedicated to generating a high-quality, complete genome for all European species, with 20 percent of those species being at risk of extinction. Here, Agilent Technologies shows how the 4150 TapeStation System is supporting this research.


Application NoteLife Sciences

Quality control in Illumina sequencing workflows using the TapeStation system

In this application note, Agilent Technologies describes quality control (QC) steps in various Illumina next-generation sequencing (NGS) workflows provided as a service by the German Cancer Research Center (DKFZ) Genomics and Proteomics Core Facility. The QC steps can be performed using the Agilent 4200 TapeStation system with the ScreenTape portfolio. Appropriate reference samples are included as positive controls to monitor the individual steps during library preparation. Representative QC data of these reference samples serve as positive examples for successful completion of critical steps of the most frequently used NGS library protocols.


Application NoteLife Sciences

Automated nucleic acid sample QC in NGS workflows

High-quality DNA and RNA samples support strong next-generation sequencing (NGS) results. To help prevent suboptimal sequencing data and avoid wasting time and resources, quality control (QC) steps are recommended at various points in the NGS workflow, from initial sample assessment to final library qualification for NGS library preparation protocols. Here, Agilent Technologies explains how the benefits of the Agilent TapeStation systems and ScreenTape technology offer ideal and easy-to-use sample QC solutions for NGS workflows.

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