GlycoWorks RapiFluor-MS N-Glycan Kit
Reimagine Released N-Glycan Analysis.
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Valuable kit.
Biosimilars
The kit is easy to handle, just need to take some precautions but it works fine. The process of cleaning and labeling takes around 15 min.
Review Date: 4 Jun 2019 | Waters
Very good product for N-linked glycan analysis by HILIC-FLR-MS
N-linked glycan analysis for therapeutic protein characterization
Ease to use, strong FLR and MS signal for glycan ID and quantification.
Review Date: 27 Jun 2017 | Waters
Easy and high throughtput kit, but it can not be applied as it is for all the proteins
Glycoprotein characterization and glycoprofiles monitoring
The kit RapiFluor is an amazing solution for easily monitoring glycoprotein. As it is supplied, it is easy, quick and truly high throughput, as the small amount of sample is necessary. All the above combined with high fluorescence and MS signals. It is the best solution for monitoring the glycoprofiles batch to batch, with the support of MS info as provided by QDA detector. The sample preparation works well for a simple mix of glycans but it has to be optimized in case of complex protein (not antibody as IgG1) with big and very complex glycans and/or high sialylated glycans. In these cases the complete glycan release and the total recovery can be not easy. The usually hard identification of small peaks, despite the amount of starting protein is less, could be easier than in the past because of the high intensity of MS signals plus the GU values for RapidFluor in UNIFY platform.
Review Date: 24 Feb 2017 | Waters
Really good, by far better than anything else available on the market at the moment!
Glycoprofiling
Amazing product! Really simplifies the work flow (you can process 24 samples in less than half a day), extremely high sensitivity and resolution by HILIC, MS compatible - for precise assignment of glycan peaks. Also - the final sample volume of 400uL gives the flexibility of measuring duplicates/triplicates by both HILIC and MS. It cannot be compared with 2-AB labelling, millions of times better! The price is higher than for 2-AB labelling, but the overall benefits for this product are incomparable.
Review Date: 27 Sept 2016 | Waters
Very clear results and easy to use
Analyze biologics and new glycoenzymes
The kit is very easy to use, especially following the 2-day training course Waters provided. The results are very clear and reproducible and considering all of the kit components it is very comparable with existing labels.
Review Date: 2 Sept 2016 | Waters
Thanks Waters
Glycosylation profile of mAbs.
The RapiFluor-MS N-Glycan Kit is very useful for the analyst because it has very easy preparation steps. The results which are obtained are very robust.
Review Date: 24 Aug 2016 | Waters
Great MS sensitivity.
Glycan analysis
Clearness of the protocol and speed of the reaction. Data analysis with UNIFY could be better as database is not complete and dextran ladder is used but having the standard glycan with the rapid fluo label could help for difficult glycan.
Review Date: 17 Aug 2016 | Waters
Rapifluor Kit is amazing !!!!
N-Linked glycan analysis
My key area of interest has always been Glycobiology and I have been involved in glycan analysis since 2007. During my early days of sample preparation, it was a nightmare with regard to post labeling clean-up steps using cartridges. Life was made easy when i started using the µHILIC plates in 2014 . With the introduction of Rapifluor kit glycan analysis was never so amazing. Not just the high-throughput sample prep but also the FLR sensitivity is great especially for the low abundant glycoforms . Truly this kit can change the whole approach of Glycan analysis. The availability of the commercial Rapifluor standards including high mannose is really useful.Thanks to Waters for the marvelous innovation !!!!
Review Date: 29 Jul 2016 | Waters
Great results after certain effort on optimization
Glyoproteins
The product offers a fast and easy sample preparation. Some steps have to be optimized for each protein individually (for optimal denaturation/deglycosylation). It delivers great MS signals. Glycans can be identified easily without interfering background/dirt signals even more valuable considering the low sample amounts. A small drawback is the low tolerance against higher buffer salt concentrations. Again this has to be evaluated for each protein separately. The manual as it is, is not applicable for all glycoproteins and needs further optimization for individual topics.
Review Date: 11 Jul 2016 | Waters
Very good and reliable kit.
Released Glycans on in process and DSP antibodies
Extremely reproducible, using can be confusing, the names 'reagent 1,2,3,4'are are very confusing to me, as it is all the same. Also it should be indicated clearer if it is a box for 24 or 96 or 3 times 8. Finally, it would be good if there was a pipetting boat for all volumes to be added, especially the enzyme is a pain (1.2 microliter) when using multichannel pipet. The enzyme solution could be better 10 times diluted anyway.
Review Date: 11 Jul 2016 | Waters
Waters has developed the GlycoWorks RapiFluor-MS N-Glycan Kit. This technology enables unprecedented fluorescent and mass spectrometric performance for glycan detection while also improving the throughput of N-glycan sample preparation.
Streamlined Workflow
GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time consuming sample preparation.
- Easy-to-follow protocol
- Detailed tips and tricks provided for adaptation
- Easily train non-glycans experts
Fast Sample Preparation
The entire process, from glycoprotein to ready-to-analyze sample, can now be completed in less than 30 minutes*. This is made possible by fast deglycosylation combined with instant labeling.
- Increase lab efficiency
- Quickly analyze screen samples and provide feedback in a timely manner
Unprecedented Sensitivity
RapiFluor-MS is a reagent comprised of an NHS carbamate rapid tagging group, an efficient quinoline fluorophore, and a highly basic tertiary amine for enhancing MS ionization. As an NHS carbamate, unlike alternative rapid tagging reagents, it hydrolyzes over time to generate carbon dioxide and a corresponding amine. This makes RapiFluor-MS labeling a self-quenching reaction.
Enhanced chemical properties for glycan analysis:
- Rapid tagging
- Efficient fluorescence
- Enhanced ionization efficiency
Increase productivity for N-linked glycan analysis of biosimilars
This application note demonstrates how the BioAccord can increase confidence and productivity in released glycan analysis for the development of biosimilars.
Best Practices in Analysis of RapiFluor-MS Labeled Glycans Using ACQUITY QDa Detector
This whitepaper provides basic understanding and guidance in the configuration of an ACQUITY UPLC System - specifically an H-Class Bio - equipped with an FLR and ACQUITY QDa Detector in the analysis of RapiFluor-MS labeled glycans. The fundamentals of mass detection theory, system configuration and method settings are discussed.
Absolute Quantitation of RapiFluor-MS Labeled N-Glycans by Calibration of Fluorescence Response
Glycosylation plays an important role in the structure and function of proteins, and altered glycosylation is implicated in numerous diseases such as cancer, autoimmune diseases and neurodegenerative diseases. In this application note, Waters presents a new method for the preparation and analysis of N-glycans and shows how the RapiFluor-MS Quantitative Standard can be used to calibrate fluorescence response and absolute quantitation.
Rapidly Monitor Released N-Glycan Profiles During Process Development
This application note describes how the RapiFluor-MS and the ACQUITY QDa Detector from Waters can reduce the sample preparation time for rapidly labeling released N-glycan and improve fluorescence signal. Using the combination of the RapiFluor-MS and ACQUITY QDa detector provides data rapidly and efficiently.
Rapid Preparation of Released N-Glycans for Hilic Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent
A team from Waters explain their new method for fast detection and fluorescent tagging of N-glycans showing how it is more efficient than the current method.
Robustness of RapiFluor-MS N-Glycan Sample Preparations and Glycan BEH Amide HILIC Chromatographic Separations
N-glycosylation of proteins is routinely characterized and monitored because of its significance to the detection of disease states and the manufacturing of biopharmaceuticals. Glycosylation profiles are most often assessed by means of released glycan analyses, wherein samples are often prepared by techniques that are notoriously time-consuming or lead to compromises in MS sensitivity. This application note discusses the attributes of RapiFluor-MS based sample preparation and the corresponding HILIC-based LC analyses.
Transferring RapiFluor-MS Labeled N-Glycan HILIC Separations between UPLC and HPLC
This application note demonstrates that Glycan BEH Amide Columns packed with 1.7 μm and 2.5 μm particle sizes afford scalability between RapiFluor-MS labeled glycan separations performed under UPLC and HPLC-compatible conditions. Using standard LC method transfer principles to account for differences in particle diameter (dp), column length, and column internal diameter, it is shown that comparable chromatographic profiles and relative quantitation can be achieved.
Biopharmaceutical Solutions: A New Way to Look at Biomolecules
Download this application note to see how Waters provides solutions to current biopharmaceutical issues. By 2014, five of the top five - and 50% of the top 100 - best-selling branded drugs will be proteins. Is your organization positioned to be one of the biopharmaceutical leaders?
Exclusive Interview with Scientists’ Choice Awards® Nominees
Hear from some of the nominees for the Best New Drug Discovery Product of 2015. Runner-up products included the Analytik Jena CyBio® Overture Liquid Handling Software, Bruker Daltonics MALDI PharmaPulse MALDI-TOF System, Promega Corporation GloMax® Explorer Multimode Detection System, Tecan Spark™ 10M Multimode Microplate Reader and Waters GlycoWorks RapiFluor-MS N-Glycan Kit.
Reimagine N-Glycan Analysis with GlycoWorks RapiFluor-MS N-Glycan Analysis Kit
Watch this video to see how the novel GlycoWorks RapiFluor-MS reagent streamlines released N-glycan sample prep and enables mass detection.
Waters' ACQUITY QDa Provides Complete Solution for N-Glycan Analysis
Watch this video to hear Rohit Khanna, VP of Marketing at Waters Corporation, describe the demand for complete innovative solutions that maximize efficiency, technology and software capabilities. An example includes fast, easy analysis of N-Glycans without compromising on sensitivity using the ACQUITY QDa system. Such mass spectrometry innovations have now become available to new market areas such as food, environmental and clinical. Interview filmed by SelectScience at Pittcon 2015.
Reimagine N-Glycan Analysis with Waters
Learn how to do complete sample preparation for released N-Glycans in just 30 minutes with three simple steps.
Webinar Highlights: Get Automated - Achieve Your Automation Potential for Released N-Glycans
Discover a new rapid, efficient and reproducible solution for a traditionally complicated method: released N-glycan profiling.
Free Webinar: Achieve Your Automation Potential for Released N-Glycans
Powerful technologies to streamline your N-glycan profiling
New Alliance Brings Enhanced Sample Preparation for Drug Development Scientists
Firms to co-market new semi-automated sample preparation approach for protein therapeutics glycosylation profiling
Waters Unveils New Line of Calorimeters, New Solid-Core Column Chemistries and More at Pittcon 2016
Company also spotlights how its glycan analysis kit has saved biopharmaceutical industry nearly four years of lab work
Thermo Fisher Scientific Wins the Scientists’ Choice Award® for Best New Drug Discovery Product of 2015
Discover the winning product, voted for by scientists worldwide
Waters GlycoWorks RapiFluor-MS Kit Now Available in a 24-Sample Format
Smaller format for fast and highly sensitive released N-glycan analysis for LC and LC-MS workflows
Vote for the Best New Drug Discovery Product of 2015 in the Scientists’ Choice Awards®
Which product made the most difference to drug discovery research in 2015?
Waters New HILIC-based Glycoprotein Column Enhances Glycan Analysis
Provides glycan occupancy information for biotherapeutic glycoproteins
What's New from Waters
Discover the innovate technologies showcased at the Waters booth at Pittcon 2015
