Absolute Quantitation of RapiFluor-MS Labeled N-Glycans by Calibration of Fluorescence Response
7 Nov 2017Glycosylation plays an important role in the structure and function of proteins, and altered glycosylation is implicated in numerous diseases such as cancer, autoimmune diseases and neurodegenerative diseases. In this application note, Waters presents a new method for the preparation and analysis of N-glycans and shows how the RapiFluor-MS Quantitative Standard can be used to calibrate fluorescence response and absolute quantitation.
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RapiGest™ SF Protein Solubilization Reagent
WatersThe patented RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N, Glu-C, PNGaseF and other enzymes.Compatible for LC and MS analysisImproves efficiency and speed of digestionDegraded at low pH and removed from the sampleSimplifies preparation protocols and improves throughput of analysisUS Patent No: 7,229,539 and US 8,580,533