G:BOX Chemi XX6

This application note outlines a method for fluorescence-based detection of proteins on Western blots. Using fluorescence-based fluorophores increases sample throughput and can make multiplexing several fluorophores less time consuming.

This application note provides a brief overview of SafeView dye from NBS Biologicals, a nucleic acid stain used in gel electrophoresis for the detection of double-strandedA (dsDNA), single-stranded DNA (ssDNA) or RNA.

This application note provides an overview of ethidium bromide dyes, commonly used in agarose gel electrophoresis to detect double-stranded DNA (dsDNA) and single-stranded DNA (ss) or RNA.

This application note provides an overview of Alexa Fluor dyes, a series of fluorescent dyes produced by Molecular Probes that span the visible spectrum.

Learn how Syngene image capture system,s combined with GeneSnap software are the perfect combination for imaging SYBR dyes.

The commonly used western blotting substrates are luminol-based and produce a chemiluminescent signal. Substrates such as ChemiFast are highly sensitive, enhanced chemiluminescent substrates. In this application note, learn how the ChemiFast substrate’s extremely intense signal output enables detection of HRP using cooled charge-coupled device (CCD) camera imaging methods.

Some manufacturers use a Flat Field Correction method in order to address uneven illumination of light sources. This involves subtracting the image information from an empty field of view or ‘perfectly flat’ fluorescent reference sample from that of the same field of view with the gel added. As this process involves the subtraction of one image from another, the integrity of the raw data of the initial captured image is compromised. Such manipulation is incompatible with Good Laboratory Practice (GLP). Syngene uses the Dynamic Fielding Correction method to address uneven light illumination whilst maintaining GLP compliance.

Western blotting is a commonly used technique to identify and quantify specific protein(s). The commonly used western blotting substrates are luminol based and produce a chemiluminescent signal. Substrates such as ChemiFast are highly sensitive enhanced chemiluminescent substrates. This application note presents ChemiFast substrate’s extremely intense signal output, enabling detection of HRP using cooled charge couple device (CCD) camera imaging methods.

Traditionally, radioisotopic protocols have always been standard in the laboratory. But with costs and concerns associated with using and disposing of radioisotopes escalating, many researchers are turning to non-isotopic methods wherever possible. Consequently, new chemistries are being developed to substitute radio-isotopic methods. The most popular method currently is that of chemiluminescence although some scientists prefer chemifluorescence. This application note defines the two techniques and offers guidance on how to select the most suitable for your research.

Chemiluminescent western blotting is the most frequently used method for detecting proteins. Recently there has been a trend towards chemifluorescence which provides the user with many advantages including shorter exposure times and also means the signal is stable for weeks. This application note will compare the performance of x-ray film with that of Syngene’s G:BOX Chemi IR6 imaging system for the visualisation and quantification of chemiluminescent and chemifluorescence signals on western blots.

Capturing chemi blot images is tricky, which is why Syngene have redesigned their GeneSys software for G:Box Chemi systems

New G:BOX systems feature super-sensitive lens for quick, accurate imaging of all gels and blots

Research is helping identify biomarkers of Alzheimer’s Disease