Chemiluminescent Western Blot Imaging using ChemiFast Substrate
20 Jul 2016Western blotting is a commonly used technique to identify and quantify specific protein(s). The commonly used western blotting substrates are luminol based and produce a chemiluminescent signal. Substrates such as ChemiFast are highly sensitive enhanced chemiluminescent substrates. This application note presents ChemiFast substrate’s extremely intense signal output, enabling detection of HRP using cooled charge couple device (CCD) camera imaging methods.
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G:BOX Chemi XX6
SyngeneG:BOX Chemi XX6 gel imaging system has a high resolution camera for imaging multiple sample types and sizes, from fluorescence 1D to 2D gels to chemiluminescent blots. Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot.