Products & ReviewLife Sciences

G:BOX Chemi XT4

For high performance imaging, the new generation of G:BOX Chemi gel doc systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it. The G:BOX Chemi series feature…

Syngene

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Synthetic Biology

The device is practical, affordable and made our lab completely self sufficient in terms of molecular DNA/Protein techniques. The software is simple and easy to use.

Review Date: 8 Oct 2014 | Syngene

For high performance imaging, the new generation of G:BOX Chemi gel doc systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it.

The G:BOX Chemi series features cameras with resolutions of 4m, 6m or 9m pixels. All have cooling which reduces the small amount of electronically generated noise to produce outstanding images with minimal background interference. These new generation cameras also have higher quantum efficiencies (QE) than most, helping the system to achieve outstanding levels of sensitivity.

Whether using the system for standard fluorescence applications such as DNA, or for chemiluminescence imaging, the G:BOX Chemi will give you high quality results. In addition, by using the optional LED modules users can work with a unique automated multiplexed function capable of using up to 5 channels. Other LED modules can be used to turn the G:BOX Chemi into a highly sensitive IR capture system. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels.

The stylish design with modular construction includes motor-driven lenses, filter wheels with the option of including a range of lighting choices for both Epi and transillumination applications. Syngene has just introduced the new high intensity HI-LEDs option for the G:BOX range, resulting in superior imaging performance for your fluorescence applications.

Check the status of an image capture and access your recently captured images from any computer, smartphone or tablet on the same network as G:BOX Chemi XT4 gel doc system using the new "Status Link" feature. This is ideal for any busy laboratory.

The system comes complete with unlimited copies of GeneTools analysis software.

Application NoteLife Sciences

Multiplexing LI-COR IRDye 680LT and IRDye 800CW

This application note outlines a method for fluorescence-based detection of proteins on Western blots. Using fluorescence-based fluorophores increases sample throughput and can make multiplexing several fluorophores less time consuming.


Application NoteLife Sciences

Imaging SYPRO Ruby and Deep Purple Stained Gels with G:BOX ichemi XR and XT

This paper denotes how SynGene’s G:BOX iChemi XR/XT multi-functional image analysis systems can be used to detect and analyse the sensitive protein stains, Deep Purple and SYPRO Ruby. SYPRO Ruby is a protein stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). SYPRO Ruby stain can be used with either 1D or 2D gels. The SYPRO Ruby stain has equivalent, or better, sensitivity than silver staining techniques. Deep Purple stain is a fluorescent stain used in the detection of proteins in gels or blots following electrophoretic separation. Deep Purple stain has been shown to be compatible with mass spectrometry.


Application NoteLife Sciences

SafeView Dye: An Overview

This application note provides a brief overview of SafeView dye from NBS Biologicals, a nucleic acid stain used in gel electrophoresis for the detection of double-strandedA (dsDNA), single-stranded DNA (ssDNA) or RNA.


Application NoteLife Sciences

G:BOX iChemi XR & XT forImaging Ribonuclease Protection Assays

Gene Expression and protein activity within a cell are of great interest to researchers. Changes in cellular mRNA levels are of particular interest as these changes directly correlate in their corresponding protein levels, there are however exceptions to the rule. Many scientists study gene expression by monitoring the response of mRNA molecules to genetic manipulations this is often accomplished by using a number of different techniques including Northern blotting, RTPCR and nuclease protection assays.


Application NoteLife Sciences

Overview of Ethidium Bromide Dyes

This application note provides an overview of ethidium bromide dyes, commonly used in agarose gel electrophoresis to detect double-stranded DNA (dsDNA) and single-stranded DNA (ss) or RNA.





Application NoteLife Sciences

Chemiluminescent Western Blot Imaging Using ChemiFast Substrate

The commonly used western blotting substrates are luminol-based and produce a chemiluminescent signal. Substrates such as ChemiFast are highly sensitive, enhanced chemiluminescent substrates. In this application note, learn how the ChemiFast substrate’s extremely intense signal output enables detection of HRP using cooled charge-coupled device (CCD) camera imaging methods.


Application NoteLife Sciences

Multiplexing LI-COR® IRDye® 680LT and IRDye® 800CW Using the G:BOX Chemi and PXi Imaging Systems from Syngene

This application note demonstrates that users can successfully multiplex between LI-COR dyes IRDye 680LT and IRDye 800CW using Syngene’s G:BOX Chemi and PXi imaging systems. One of the main advantages of using a G:BOX Chemi and PXi system is the faster imaging exposure times compared to that of the Odyssey Infrared Imager and also that the G:BOX Chemi and PXi systems offer great versatility enabling the imaging system to be used for a variety of different applications.





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