FlowCellect™ Human NK Cell Characterization Kit
The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-colo…
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The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-color tests. Detailed assay instructions are included to assist in analysis and to ensure the correct cell concentration is obtained during acquisition of sample data.
Identification and Functional Characterization of Natural Killer Cells Using Flow Cytometry
Natural killer (NK) cells are a subset of lymphocytes involved in innate immunity. Their mechanisms of action include the direct lysis of non-self target cells through release of perforin and granzymes, as well as the production of cytokines that are supplied to other innate immune cells. This application note describes the use of a novel flow cytometry kit to characterize NK cells in human peripheral blood and the use of flow cytometry to perform a cell cytotoxicity test to detect NK-mediated killing of human K562 myeloma cells.
