Identification and Functional Characterization of Natural Killer Cells Using Flow Cytometry
29 Jul 2013Natural killer (NK) cells are a subset of lymphocytes involved in innate immunity. Their mechanisms of action include the direct lysis of non-self target cells through release of perforin and granzymes, as well as the production of cytokines that are supplied to other innate immune cells. This application note describes the use of a novel flow cytometry kit to characterize NK cells in human peripheral blood and the use of flow cytometry to perform a cell cytotoxicity test to detect NK-mediated killing of human K562 myeloma cells.
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FlowCellect™ Human NK Cell Characterization Kit
MerckThe FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-color tests. Detailed assay instructions are included to assist in analysis and to ensure the correct cell concentration is obtained during acquisition of sample data.
Guava® easyCyte™
LuminexOur microcapillary flow cytometry systems are simpler to operate than traditional sheath-fluid based instruments and are far easier to maintain. They utilize small sample volumes, generate minimal waste, and have lower operating costs. As a result, easyCyte™ Flow Cytometers are uniquely amenable to on-demand use in the laboratory environment and have helped many scientists achieve insightful cellular analysis since 1998.