CytoFLEX Flow Cytometer

Explore the fascinating history and groundbreaking discoveries in the field of extracellular vesicles (EVs). In this infographic, we take you on a journey starting from the early days of EV research and the unexpected role of EVs in blood clotting. Discover how scientists gradually unraveled the enigmatic functions of EVs, leading to a deeper understanding of their critical roles in cellular processes. Understand the ongoing efforts to navigate the remaining gaps in EV research and their promising potential in disease diagnostics and targeted therapies. Uncover how these tiny vesicles are paving the way for innovative medical advancements.

An expert guide for harmonizing workflows, enhancing efficiency, and enabling reliable data.

Clinical trials demand precision, coordination, and scalable workflows. This eBook explores how partnering with an end-to-end solutions provider in flow cytometry can help overcome common trial challenges—from multicenter harmonization and ethical compliance to data management and automation.

Whether you’re a principal investigator navigating complex datasets, a lab manager maintaining regulatory excellence, or a clinician focused on patient outcomes, this guide offers practical insights for improving workflow standardization and research reproducibility.

Download the guide to explore:

• Best practices for harmonizing multicenter trials
• How expert partnerships improve data quality and efficiency
• The role of automation in sustainable, scalable research workflows

Equip your lab to lead in clinical research—with insights that drive real-world impact.

Rare event cytometry faces the challenge of abundancy of non-target events and heterogeneity that may overlap with hallmark features of the target cells. However, this challenge can be resolved by using highly discriminative antibody panels with high-recovery gating strategies, robust plausibility checks and effective gate purification steps. Such a methodology is demonstrated in the application note below.

Escherichia coli are bacteria which can be found in foods, the environment and within the intestine of living organisms. Particular strains of E.coli can cause illness. In this application note, a quick protocol to enumerate bacteria in a sample using the CytoFLEX flow cytometer is presented. The range of resolution can support the identification of bacteria by forward and side scatter parameters and does not require any fluorescent dyes or counting beads to enable detection or enumeration.

Peripheral blood dendritic cells (PBDC) can be subdivided into two main cell types, namely myeloid DC (MDC) or DC1, and plasmacytoid DC (PDC) or DC2. PBDC are defined as lineage negative cells, i.e lacking markers for T lymphocytes, B lymphocytes, NK cells and monocytes/macrophages, while expressing high levels of HLADR. However, the lack of consensus on what markers should be used in this complicated “lineage” cocktail is an issue for comparison among studies, and raises the need for a more consensual approach to PBDC identification. This poster demonstrates a simple approach for MDC and PDC identification in whole blood samples using the restricted expression pattern of the ILT3 molecule.

This application note describes immunophenotypic analysis of human peripheral blood lymphocytes utilizing a 6-color antibody panel in a stain/lyse protocol run on the CytoFLEX Research Flow Cytometer. The markers chosen are commonly used surface CD antigens which can be used to quantify and characterize normal and abnormal lymphocyte populations in peripheral blood.

Performance testing of cytometry instruments is utilized to characterize the capability of the cytometer to perform high complexity applications. Presented in this scientific poster is the performance analysis of the CytoFLEX flow cytometer, tested against measurements for sensitivity, dynamic range, population resolution, linearity, stability, reproducibility, and small particle resolution. Commercially available bead standards were used to evaluate performance expectations across multiple parameters.

Multi-color flow cytometry is a powerful tool to analyze the highly heterogeneous human T cell compartment. This application note defines a 13-color tube which allows for the identification of major peripheral T cell subsets according to classical and more recent characterization criteria, with a minimum of sample preparation effort. T lymphocytes (T cells) form an essential part of the adaptive immune system and are therefore of major interest for the research community.

This application note explains the set-up procedure for use of a new proprietary dry reagent formulation that secures long-term fluorochrome stability while stored at room temperature with the CytoFLEX flow cytometer. Tandem dyes play an important role in high content flow cytometry. However, degradation of tandem dyes over time due to light and oxygen exposure as well as inappropriate storage conditions can severely affect their emission spectra and may result in the need for frequent compensation adjustment. Furthermore, manufacturer dependent spectral variability may occur in tandem dyes that could require lot-specific compensation settings.

This webinar explores the limits of flow cytometry technology and outline how the CytoFLEX nano flow cytometer is designed to help address these limitations in a robust and consistent system able to standardize results between labs. Our expert speaker, Alfonso Blanco of University College Dublin, will discuss the advantages of using fluorescence combined with multiple scatters for further characterization of nanoparticles and extracellular vesicles. In addition, he will present some preliminary results research groups have obtained with the CytoFLEX instrument.

Key learning objectives

• Discover the current limits for flow cytometry
• Gain an introduction to the CytoFLEX nano flow cytometer
• Explore samples run

Who should attend?

Core lab managers, principal investigators, research scientists, core facility managers, data scientists, analysts, professors, instructors, researchers, postdoctoral research fellows, laboratory assistants, laboratory coordinators, laboratory specialists, group leaders

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Instrument breaks the boundaries of EV detection, providing access to new data points

An automated gating workflow can shorten hands-on time by up to 75%

Simple, powerful user experience combines with reliability and scalability allowing users to focus on experimental results instead of process

Evaluating the immune status of people suffering from leishmaniasis to facilitate developing a therapeutic vaccine

Beckman Coulter to present at the European Society for Clinical Cell analysis (ESCCA) 2017

Joins Dr. Beckman and Wallace Coulter as Fellows of American Institute for Medical and Biological Engineering (AIMBE)

Most interesting new application articles from the SelectScience® library

Renowned gene therapy research team from the UCL Cancer Institute awarded a CytoFLEX Flow Cytometer

Visit Booth #1010 to for in-booth presentations and to meet the winners