Counting Eschericia coli Using the CytoFLEX Research Flow Cytometer

Escherichia coli are bacteria which can be found in foods, the environment and within the intestine of living organisms. Particular strains of E.coli can cause illness. In this application note, a quick protocol to enumerate bacteria in a sample using the CytoFLEX flow cytometer is presented. The range of resolution can support the identification of bacteria by forward and side scatter parameters and does not require any fluorescent dyes or counting beads to enable detection or enumeration.