CLARIOstar® Plus Multi-mode Microplate Reader

Ferroptosis is an iron-dependent form of cell death driven by oxidative damage to membrane lipids, implicated in neurodegenerative diseases. Reactive oxygen species (ROS) generated during hypoxia-reoxygenation, via the mitochondrial NCLX sodium/calcium exchanger, promote lipid peroxidation and cell death. Here, the CLARIOstar Plus microplate reader was used to measure the inhibition of lipid peroxidation in a fluorescence–based lipid peroxidation assay while changing oxygen levels with the Atmospheric Control Unit (ACU).

Discover how THUNDER™ TR-FRET assays on the CLARIOstar Plus enable fast, sensitive, and cost-effective detection of phosphorylated proteins like ERK1/2, p38αβγ, and STAT3 in whole-cell lysates for high-throughput cellular kinase research.

In this eBook, BMG LABTECH delves into the current microplate-based assays utilized in virology research, uncovering the novel insights these assays offer and their impact on the field. The recent COVID-19 pandemic highlighted the profound impact viruses can have on health, society, and the economy, reigniting interest in virology. Modern virology aims to understand and characterize viruses for diagnostic tests, antiviral drugs, and vaccines. Microplate-based assays and plate readers have become indispensable tools in this pursuit, providing researchers with flexible and efficient means to gather objective and reproducible data for monitoring viral infections in real-time.

In this eBook, BMG LABTECH discusses microplate-based assays in microbiology research, covering the use of microplate readers in microbiology research applications. It highlights the advantages of using microplate-based assays for monitoring microbial growth and viability, functional assays, and high-throughput screening for antimicrobial compounds. The eBook explores different types of microplate-based assays that can be used in microbiology research and provides application notes on various assays, including growth, detection, and monitoring of bacteria, yeasts, and viruses. The eBook serves as a comprehensive guide for microbiologists looking to leverage the benefits of microplate-based assays in their research, and is recommended for those who want to perform time and cost-efficient microbiology assays.

In this application note, BMG LABTECH introduces the HiBiT CETSA, a method for measuring protein-ligand binding in a tracer-free manner. This approach can improve the screening for new ligands by allowing the measurement of endogenous full-length proteins. The HiBiT CETSA detects interactions between proteins and potential ligands by determining the protein's melting point along a heat gradient. The assay allows plotting the temperature against the detected luminescence, and subsequently, the melting curves are analyzed to identify compound-induced thermal shifts.

In this application note, BMG LABTECH discusses the limitations of conventional microplate readers when it comes to temperature stability and its effects on assay precision and reliability. The PHERAstar® FSX with Advanced Assay Stability (AAS) system eliminates external air flow to the inside of the reader, maintaining constant humidity and reducing evaporation. The AAS system enables the PHERAstar FSX to match room temperature conditions and to be maintained at any temperature between 18° and 45°C. It achieves this by automatically heating or cooling the microplate measurement chamber depending on the external environment.

In this application note, BMG LABTECH discusses the use of microplate readers for measuring the motility of cells, specifically in the context of wound healing and invasion assays. The note describes important factors to consider when performing these assays, such as the choice of a proper cell line, the selection of the right assay format, and the attainment of proper cell confluency prior to the assay. The note also discusses the use of key detection technologies such as fluorescence, luminescence, and absorbance, comparing the benefits and drawbacks of each technology.

In this application note, BMG LABTECH describes the development and validation of a cell-based mode-of-action assay, by which the total nonsteroidal anti-inflammatory drug (NSAID) activity in a wastewater sample enriched by solid phase extraction (SPE) can be measured as equivalents of the lead substance diclofenac (DicEQ).

In this application note, BMG LABTECH describes three different miniaturized experiments that enable users to identify and rapidly reduce cross-talk using the example of the BacTiter-Glo™ assay.

There is an almost dizzying range of cell-based assays now available such that researchers can find it challenging to select the right measurement technology for their needs, and to stay informed about current trends and new technology options. Microplate readers and their add-ons can provide a valuable alternative to microscopes for researchers and drug developers looking to get the best results quickly, and in real time, at an affordable cost.

This eBook explores different applications supported by cell-based assays to support you in selecting the right ones, and to demonstrate where microplate readers could provide value to your workflow.

Download for content including:

• Transfection efficiency
• Expression and regulation of BRET donor-labelled proteins
• Real-time measurement of viral gene expression
• Cell viability and apoptosis assays
• Viral cytopathic effects
• Versatility in long term cell-based assays
• Real-time calcium flux measurements
• GPCR activation measurements
• Phospho-ERK1/2 assay
• Analysis of cell migration
• Assay detects residual NSAIDs
• Ischemia-reperfusion conditions

Discover tips and considerations for improving microplate reader measurements. Application specialists, Dr. Ann-Cathrin Volz and Dr. Martin Mangold from BMG LABTECH, discuss the impact of different plate reader parameters on data, and provide empirical evidence of their effect on data quality. They also discuss approaches to optimize microplate reader settings, and share recommendations for using BMG LABTECH's range of microplate readers.

In this video, Eric Matthews, Vice President of Sales at BMG LABTECH USA, discusses the features to look out for when looking to purchase a new microplate reader. Plus, Matthews explores what technologies contribute to reader performance and when trade-offs between performance and flexibility may be worthwhile.

In this video, Eric Matthews, Vice President of US Sales and Business Development at BMG LABTECH, discusses its comprehensive portfolio of microplate readers and how this is designed to offer easy-to-use, high-quality microplate readers for a range of different life sciences applications.

In this video BMG Labtech introduce the CLARIOstar Plus, a multi-mode microplate reader for assay development. LVF Monochromators™, highly sensitive filters, and an ultra-fast UV/vis spectrometer are designed to ensure flexibility combined with the best sensitivity of its class. The enhanced dynamic range and automatic focus technologies make manual settings easier.

In this presentation at SLAS2020, senior application scientist Carl Peters, from BMG LABTECH, and Erik Schaeffer, Co-Founder, President & Chief Scientific Officer at AssayQuant Technologies, Inc., discuss the exciting area of protein kinases as a target class, highlighting an effective new approach to monitoring and assessing the mechanism of kinases and phosphatases and the microplate reader technology supporting this collaboration.

This presentation was filmed at SLAS2020 - watch other highlights from the show here >>

In this video, watch Dr. Ben Allsop's presentation on simulating ischaemia-reperfusion injury in vitro to find new mechanisms of protection and prevention. Plus, hear Catherine Wark, Applications Manager of BMG Labtech, discuss how the new features of the CLARIOstar PLUS make it ideal for these types of assays.

This presentation was filmed as part of our coverage of SLAS2019 - see other video highlights here >>

Understanding disease at the molecular level will enable novel, more effective compounds to be developed. This workshop presentation illustrates how, using the new CLARIOstar PLUS multi-mode microplate reader, scientists are able to track, and evaluate molecular kinetics in real-time.

This interview was filmed as part of our coverage of SLAS2019 - see other video highlights here >>

Prince Saforo Amponsah, PhD Student at the University of Kaiserslauten, is interested in redox biology and its effect on cellular physiology. In this video, Amponsah discusses how using the CLARIOstar® microplate reader in his research enables highly sensitive detection of changes in subcellular redox status, in medium to high throughput. He describes how the ability to adjust bandwidths with the LVF monochromator enables detection of many sensors in one run. In the future, this could enable multiple redox measurements across subcellular compartments, in order to understand redox cross-talk.

George Kosmidis, scientist at Ncardia, is involved in the fascinating field of stem cell biology; specifically the use of stem cell derived cardiomyocytes as a platform for drug efficacy and toxicity screening. In this video, Kosmidis describes how the CLARIOstar® is integral to his research. Using this advanced monochromator microplate reader he is able to analyse DNA damage, mitochondrial content and ROS species. He also explains how the powerful software analysis platform truly sets the CLARIOstar apart.

Carl Peters, senior applications scientist, BMG Labtech, and Terry Riss, global strategic marketing manager, cell health, Promega, present streamlined multiplexing of cell assays and improved assay data with BMG Labtech’s CLARIOstar. CLARIOstar is a multimode microplate reader with an integrated atmospheric control unit, as well as a revolutionary LVF monochromator for even greater sensitivity. Riss explores methods to optimize multiple cell assays so as to accelerate your research with real-time technology.

This special feature explores the latest research, applications, and advances in cell-based assay technology

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SelectScience goes behind the scenes to meet some of the key people driving the microplate reader innovator