Air-Dryable Probe 1-Step Mix
Air-Dryable Probe 1-Step Mix is a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA or DNA sequences.
Designed for rapid air-drying
Stability after 12 weeks at 37 °C
Reliable performance in multiplex setup
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Designed for rapid air-drying, this qPCR mix enables dried assay formats using a standard laboratory oven, offering a simpler, faster, and cheaper alternative to lyophilisation.
The Air-Dryable Probe 1-Step Mix includes a glycerol-free 4x qPCR mix containing hot start Taq polymerase, dNTPs, Mg and a blend of excipients to ensure reliable air-drying, without loss of activity. The mastermix also contains a novel version of UltraScript® Reverse Transcriptase, modified to increase stability upon drying, and our RiboShield® RNase inhibitor, so the final blend is suitable both for qPCR detection of DNA and 1-Step RT-qPCR for detection of RNA targets.
Features
- Unbiased, sensitive detection of both DNA and RNA targets, 4 copies per reaction
- Complete 1-step RT-qPCR mix, single tube format
- Easy reaction set up, add only primers & probes before drying
- Rapid air-drying protocol, 80-90 min drying time
- Same high performance before & after drying
- Room temperature storage and shipping of dried reactions
- Increased sample volume input
Key benefits of air-drying
- Reduced volume and mass of reaction mixes leads to cheaper shipping and storage.
- Room temperature stability of the air-dried final gel eliminates the need for cold-chain shipping and storage. This simplifies logistics and reduces overall cost.
- Increased volumes of sample can be used. The sample input volume can equal the final reaction volume. This is perfect for dilute samples (with a low target copy number).
- Air-drying in an oven is a highly accessible method, because there is no requirement for specialist equipment and knowledge, eliminating the need for additional staff or project out-sourcing
- Increased control over the drying process and final product development for small and medium sized IVD kit developers
Advancing molecular biology research: Innovations and collaborations at PCR Biosystems
In this video, Dr. Shane Sanassy, an International Sales Manager for PCR Biosystems, and Dr. Matteo Beretta, a Senior Molecular Biologist and Commercial R&D Director for PCR Biosystems, discuss the company’s goal of simplifying molecular biology research and assay design, and its unique product development strategy for high-quality enzymes. They introduce innovative products including the air-dryable mix for room temperature stability in IVD assays and the VeriFi Library Amplification Mix for GC bias-free NGS libraries. Beretta also explains the importance of the buffer component and PCR Biosystems’ capability in improving its composition.
New high-performance air-dryable PCR reagents make simple, cost-effective dried assay development a reality
The new portfolio of reagents offers a simpler, faster, and cheaper alternative to lyophilization
