Cloning and Expression Vectors / Systems

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qua... Read more...

Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high... Read more...

Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accele... Read more...

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream scree... Read more...

These bacterial expression vectors carry the gene for either GFP (wild type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light [360–400 nm]) and are driven by th... Read more...

Genetic engineering is the process of manipulating the genetic material of an organism — often to include the DNA from a foreign organism. Using the classic pGLO Bacterial Transformation Kit, students transform bacteria by introducing a g... Read more...

Universal, clinically applicable enhancer solution for lentivirus based transductions. Read more...

Provides helper phage function in packaging a common phage display phagemid. Infection of bacteria via pIII. Read more...

Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including m... Read more...

With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment. Read more...

The next generation of RNP reagents Read more...

The T7 Endonuclease Detection Assay is a well-known method for detecting genome editing events from CRISPR, Zinc-finger nuclease, and TALEN gene targeting. Originally identified from Escherichia coli bacteriophage, the T7 endonuclease can... Read more...

All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids for use with monocots and dicots. Read more...

CRISPR/Cas9-mediated recombineering is the most powerful bacterial genome engineering method to date. In addition, Cas9-mediated recombineering overcomes the dependence on a second recombination step, avoids the creation of destabilizing ... Read more...

This Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. Read more...

Universal negative control CRISPRs have been designed not to recognize any sequence in the human, mouse or rat genome.  Read more...

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA ® synthetic gRNA, plasmid ... Read more...

Exclusive human or mouse whole genome arrayed libraries developed with the Wellcome Sanger institute Read more...

Pooled CRISPR screening with 10x Genomics compatibility Read more...

Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments Read more...

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments. Read more...

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments. Read more...

The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018) Read more...

The Human Whole Genome Toronto KnockOut Library v3 (TKOv3) is optimized for editing efficiency using empirical data described in the findings by the Moffat lab. Read more...

The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ... Read more...