Visual ChIP Sequencing Protocol: Chromatrap ChIP-Seq Assay using Sonication for Chromatin Shearing
6 Apr 2017
This visual protocol presents step-by-step process for a successful ChIP-Seq assay using the Chromatrap ChIP-Seq kit. Learn how this robust and sensitive assay can be performed in under 5 hours in 5 steps: chromatin preparation, chromatin shearing using sonication, immunoprecipitation, reverse cross linking and DNA analysis.
About the company
Chromatrap
Chromatrap® is a product of Porvair Sciences, a wholly owned subsidiary of Porvair plc.
Chromatrap provides a revolutionary solid state platform for chromatin immunoprecipitation using spin columns. We provide faster, easier and more efficient assays to help researchers get more from their epigenetics research.
We have products to suit a range of needs, including spin columns and high throughput 96-well plates, with a range of different formats to suit your needs. These include protein A and G, sonication and enzymatic shearing. All our kits can be used for a number of downstream processes including qPCR and next generation sequencing.
