Thermo Fisher Scientific Reveals New Ion AmpliSeq HD Technology

Ion AmpliSeq HD technology is a new amplicon-based library preparation technology for Ion Torrent next-generation sequencing (NGS) that gives you the power to design your own panels and find variants with a low limit of detection—down to 0.1% for cell-free DNA samples. This revolutionary technology enables researchers to routinely get answers when ultrahigh sensitivity is required, such as when detecting low-frequency alleles in circulating tumor DNA or trace pathogenic microbial species in blood. Combined with the Ion GeneStudio S5 sequencers, Ion AmpliSeq HD panels offer a flexible targeted-NGS solution for: Cancer research Infectious disease research Microbial studies Gene expression studies How does Ion AmpliSeq HD technology work? Ion AmpliSeq HD technology leverages unique molecular tags, or UMTs, and the made-to-order design pipeline in Ion AmpliSeq Designer to help deliver results with ultrahigh sensitivity. Unmatched sensitivity for your research As genetic research rapidly evolves, the ability to confidently detect low-frequency variants or quantitate transcript-level expression is becoming more critical to the development of NGS assays with ultrahigh sensitivity. Ion AmpliSeq HD panels have been shown to achieve a 0.1% limit of detection for cell-free DNA samples, with >99% specificity for SNVs, hotspots, indels, CNVs, and fusions. Ion AmpliSeq HD panels support both DNA and RNA samples and require as little as 1 ng nucleic acids input from FFPE samples.