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Open source software for multiplex staining & image analysis

23 Oct 2024
Open source software for multiplex staining & image analysis

Dr. Sarah McArdle presents on open source software for quantitative image analysis, focusing on multiplex staining for high dimensional analysis. She explains the RareCyte Orion microscope and its image acquisition process. The discussion covers the advantages of open source software, challenges in analyzing large datasets, and the importance of validating staining quality.

Orion Multiplex Imaging Instrument

RareCyte, Inc

The Orion Platform delivers spatial biology and cellular phenotyping at a speed and resolution needed for translation from research to the clinic. It utilizes single round immunofluorescence staining and imaging of standard FFPE or fresh frozen tissue to deliver subcellular quantitation of biomarker targets across a whole slide in only a few hours. Combining speed and resolution with the additional benefits of industry-standard H&E and IHC modes, comprehensive phenotypic profiling of patient cohorts can be generated for characterization of tissue architecture, tumor heterogeneity, and the immune response. Use your own antibodies, run panels from an extensive biomarker list, or engage RareCyte services for custom panels and programs.  

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Open source software for multiplex staining & image analysis