Using extracellular flux analysis to assess T cell bioenergetic poise and spare respiratory capacity

22 Feb 2023

In this application note, learn more about the use of the Agilent XF T cell metabolic profiling kit. This new product uses an improved uncoupler (BAM15) for more consistent and accurate measurements of T cell bioenergetic capacity with less concentration optimization required than FCCP. It can be used to assess the impact of the medium composition during T cell expansion following the Agilent XF T Cell Persistence Assay workflow. This workflow allows for the evaluation of glycolysis and mitochondrial activity and capacity simultaneously, providing a comprehensive metabolic profile of T cells that can be incorporated into monitoring and improving the design and development of T cell therapies.