The power of in-line mixing for competitive compound analysis

This application note introduces NeXtStep™ injections on the Octet^® SF3 SPR system, a powerful method for performing rapid, in-line mixing competition assays without the need for pre-mixed samples. Learn how this technique simplifies assay setup, reduces false positives, and enables accurate site-specific binding analysis. Featuring real-world examples with sulfonamides, the note demonstrates how NeXtStep™ enhances fragment screening and small molecule characterization with high sensitivity and efficiency.