Single-molecule localization microscopy - Beyond the coverslip
18 Jul 2023Traditional microscopy methods have been used for imaging biological organization but are limited to a lateral resolution of ~200-300 nanometers. Single-molecule localization, a super-resolution microscopy approach, allows for high-resolution imaging of specifically labeled biological samples at a resolution of 20 nanometers laterally. Here, Bruker Fluorescence Microscopy summarizes the presentation given by Lauren Gagnon, Applications Scientist for Bruker’s super-resolution microscopy solutions. Presented here are the advantages and principles of single-molecule localization. Also, the unique benefits of the Vutara top-hat illumination and bi-plane technology, as well as the multiplexing capabilities supported by the microfluidics unit, are discussed. Finally, examples of imaging a variety of sample types and popular applications of single-molecule localization microscopy are presented.
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PlexFlo
Bruker Fluorescence MicroscopyBruker microfluidics accessory units are designed to allow sequential labeling to be incorporated into localization microscopy protocols with the Vutara VXL single-molecule localization microscope. Bruker offers two units—PlexFlo and PlexFlo96. The units are controlled by Vutara's SRX software and allow flexible configuration of fluidics sequences that are run in conjunction with imaging cycles to allow users to label an unlimited number of probes and image them in a sequential fashio
Vutara VXL
Bruker Fluorescence MicroscopyThe Vutara VXL comprehensive biological workstation for nanoscale biological imaging incorporates Bruker’s industry-leading single-molecule localization microscopy (SMLM) technology in a streamlined system with a compact footprint. The new system enables research on DNA, RNA, and proteins and supports advanced spatial biology research. When combined with Bruker’s unique microscope fluidics unit, Vutara VXL enables multiplexed imaging for targeted, sub-micrometer multiomics.