Simultaneous Quantitative and Qualitative Analysis of Proteolytic Digests of Therapeutic Monoclonal Antibodies using a TripleTOF® System

In recent years MS has emerged as a superior method for characterizing the heterogeneity of monoclonal antibodies. This application note presents a high-speed LC/MS/MS method for the analysis of enzymatically digested therapeutic antibodies. Sophisticated software tools, that enable more accurate peptide mapping, are also described.