Simplifying Microtissue-Based Screening Using Automation and the EnSight Multimode Plate Reader

Three-dimensional (3D) cell culture spans the gap between the traditional “petri dish-based” format and whole-animal systems giving unique perspectives on tumorigenesis. Process standardization is essential to enable the generation of robust and reproducible data with more biologically relevant, complex, 3D models. Here, we demonstrate how producing and characterizing 3D spheroid microtissues with the InSphero GravityPLUS™ hanging drop microplate platform can be simplified and standardized by integrating automated liquid handlers with the EnSight™ Multimode plate reader to reduce variability, hands-on time and errors produced by manual pipetting.