EnSight™ Multimode Plate Reader
The EnSight ® multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. In addition, stacker and robotic interface options are available, ideal for automation. The system’s modular design, combined with workflo…
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Very versatile instrument offering imaging on top of various homogeneous read-outs.
Multiparametric reporter gene assays
The imaging unit offers great opportunities to multiplex and/or normalize homogeneous assays. Brightfield plus various fluorescent channels offer a great variety of additional assays such as determining cell confluency e.g. to assess cytotoxicity or check for compound precipitation, or count nuclei after Hoechst staining before lysing your cells for the homogeneous assay.
Review Date: 6 Feb 2019 | Revvity
The system has a modular design that lets you add detection modes as your needs change, and combined with workflow-based Kaleido™ data acquisition and analysis software, you have a truly versatile plate reader that gets users productive quickly – making it ideal for multiuser environments.
- Alpha technology and conventional multimode detection technologies
- Easily configurable and field upgradable
- Base unit with filter-based absorbance technology and temperature control unit (up to 65°C)
- Eight barcode identified filter positions
- Integrated barcode reading from all four sides of a microplate
- Kaleido™ Data Acquisition and Analysis Software
Our Enhanced Security option for the Kaleido software provides technological controls and features that support 21 CFR Part 11 compliance. It empowers users based on their authorization level to assign access, implement data security, approve workflows with electronic signatures, and view all user actions from a comprehensive audit trail.
Technologies:
- AlphaLISA® / AlphaScreen® detection with high power laser excitation
- Fluorescence intensity detection (top- and bottom-reading) with monochromator
- Time-resolved fluorescence detection, for example DELFIA® and LANCE® technologies
- Ultra-sensitive luminescence for low signals
- Filter- and optional monochromator-based absorbance detection
Quad Monochromator Plate Reader
Our quad monochromator plate reader prevents high background when measuring fluorescent proteins, identifies peak wavelengths more accurately, and eliminates the need to exchange filters:
- For fluorescence and absorbance measurements, it enables you to select any wavelength and perform scans for the best possible signal-to-background ratio
Key Features:
- Temperature control to 65°C
- Plate barcode reader
- Plate shaking
- Plate stackers, 20 plates or 50 plates
- Robotic Interface for automation solutions
Simplifying Microtissue-Based Screening Using Automation and the EnSight Multimode Plate Reader
Three-dimensional (3D) cell culture spans the gap between the traditional “petri dish-based” format and whole-animal systems giving unique perspectives on tumorigenesis. Process standardization is essential to enable the generation of robust and reproducible data with more biologically relevant, complex, 3D models. Here, we demonstrate how producing and characterizing 3D spheroid microtissues with the InSphero GravityPLUS™ hanging drop microplate platform can be simplified and standardized by integrating automated liquid handlers with the EnSight™ Multimode plate reader to reduce variability, hands-on time and errors produced by manual pipetting.
Rapid, Reliable Measurement of Cellular Proliferation and Toxic Compound Effects on 3D Spheroid Cultures Grown from Multiple Cancer Cell Lines with ATPlite™ 3D and ATPlite 1step 3D
Cellular viability and toxicity assays are fundamental tools in the drug discovery process – used to evaluate both the potency of compounds, as well as their toxicity profiles for early drug safety assessment. Cells cultured in 3D microtissues exhibit inherent differences in cellular adhesion and require substantially stronger lysis conditions than is sufficient for traditional monolayer or suspension cultures to access the cells deeper inside each spheroid. In order to address this need, we developed ATPlite 3D and ATPlite 1step 3D assay kits specifically for use with 3D cell cultures.
Multiparametric Analysis of Cytotoxicity using Cell Imaging in Combination with Luminescence
Cytotoxicity can be caused by different types of agents, including drugs, pathogens, immune cells and external stress factors such as heat. Particularly during the drug development process, where cytotoxicity remains one of the major causes of drug withdrawal, there is an urgent need for reliable and time-saving assay workflows that gain a more complete picture of the effects of cytotoxicity. This application demonstrates how multiparametric analysis of cytotoxicity, using different technologies, can be easily performed on the EnSight™ Multimode Plate Reader and how brightfield confluency analysis serves as a robust indicator for toxicity in adherent cells and can be easily applied in labeled or label-free cell-based assay.
Cell Counting and Confluency Analysis as Quality Controls in Cell-based Assays
Learn how to determine cytometric parameters using the brightfield, digital phase and fluorescent imaging modes on PerkinElmer’s EnSight™ Multimode Plate Reader for quality control of cell-based assays.
Cell Signaling and Pathway Analysis Solutions
This video discusses the importance of cell signalling and signalling pathway analysis in drug discovery. Understanding the signalling pathway and its impact on cell pathology enables the development of new, life-saving treatments. PerkinElmer has produced a range of products that make the high-throughput analysis of cells as quick, cheap and easy as possible, without compromising results.
Leveraging High Content Phenotypic Screening to Study More Complex and Predictive Disease Models for Drug Discovery
Dr. Eberhard Krausz, from VIB, has the responsibility of translating research findings into drug discovery programs. Advocating the use of cell based screening and assay approaches to drug discovery, Dr. Eberhard discusses in this video how he first began using PerkinElmer’s instruments over ten years ago. Dr. Eberhard recommends PerkinElmer’s latest Opera Phenix High Content Screening System, suggesting it will continue to aid in future studies of complex, predictive disease models to identify new chemicals for drug discovery and development.
PerkinElmer’s Phenotypic Screening Solutions for Higher Physiological Relevance
Hear how scientists are using PerkinElmer’s HCS systems and informatics platforms to accelerate their phenotypic screening workflows and better understand disease.
Introducing the EnSight™ Multimode Plate Reader from PerkinElmer
PerkinElmer's EnSight Multimode Plate Reader is the first benchtop system to offer well-imaging alongside label-free and labeled detection technologies - for a whole new perspective on your research. This video outlines the Ensight's unique combination of industry-leading technologies, which allows you to take a truly orthogonal approach to your research and gain new insights from your assays that you could not achieve before.
The New EnSight™ - Target-based and Phenotypic Assays for an Orthogonal Approach to your Research
Watch the video to hear about the new EnSight™ Multimode Plate Reader from PerkinElmer. By offering well-imaging alongside labeled and label-free detection technologies, the EnSight™ allows scientists to combine target-based and phenotypic assays and take a truly orthogonal approach to their research.
VIDEO TOUR: ENSIGHT™ PLATE READER
Explore your research from a different perspective. Take a closer look at the features and benefits of EnSight, the first plate reader to combine multimode labelled, label-free, and image-based detection technologies in a single desktop platform.
