SFC Analytical to Preparative Scale Up of Similar Compounds with Torus Diol Columns: Separation of Benzoic Acid and Derivatives
20 Sept 2017For supercritical fluid chromatography (SFC) separations, the scaling strategy between columns of different geometries/particle sizes differ from that used for reversed phase liquid chromatography (RPLC). When scaling between columns, column pressure changes which affects the density of the SFC mobile phase. Mobile phase elution strength is altered, which if not compensated for, can mace SFC method scaling difficult. This is especially true for challenging separations such as that of benzoic acids. In this application note, Torus columns are demonstrated to provide scalable analytical to preparative SFC methods.
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ACQUITY UPC2 Trefoil and Torus Columns
WatersACQUITY UPC2 Column Technology for Chiral and Achiral Separations. ACQUITY UPC2 Trefoil™ 2.5 µm Columns are uniquely designed for the ACQUITY UPC2 System. Selectivity and speed for chiral separations Reduce method development time Based on modified polysaccharide-based stationary phases for broad-spectrum chiral selectivity. ACQUITY UPC2 Torus™ Columns simplify the method development process with four completely new and innovative 1.7 µm chemistries for convergence chromatography. Excellent peak shape and robustness while reducing or eliminating the need for additives Four column chemistries provide added selectivity for a wide range of compounds Column Specifications: Technology: Torus, Trefoil Chemistry: 1-AA, 2-PIC, DEA, DIOL, AMY1, CEL1, CEL2 Particle Size: 1.7, 2.5 µm Pore Size: 130 Å Length: 50, 75, 100, 150 mm ID: 2.1, 3.0 mm