ACQUITY UPC2 Trefoil and Torus Columns

For supercritical fluid chromatography (SFC) separations, the scaling strategy between columns of different geometries/particle sizes differ from that used for reversed phase liquid chromatography (RPLC). When scaling between columns, column pressure changes which affects the density of the SFC mobile phase. Mobile phase elution strength is altered, which if not compensated for, can mace SFC method scaling difficult. This is especially true for challenging separations such as that of benzoic acids. In this application note, Torus columns are demonstrated to provide scalable analytical to preparative SFC methods.

The development of analytical methods for the separation of chiral compounds is important in many areas of research, as it is well known that different enantiomers are selectively biologically active. It is estimated that 20 to 30% of pesticides on the market today have optical isomers, and there are reports that 40% of the pesticides used in China are chiral. This application note presents the enantiomeric and/or diastereomeric resolutions of 12 triazole fungicides using Waters® Trefoil Column Technology.

Trace impurities in synthetic products that interact with human end users or may have an undesirable environmental fate are regulated by various government agencies such as FDA and EPA. As a result, impurity separation and their structural identification are important research functions for many industries, including pharmaceutical, agrochemical, food, and consumer products. This application note describes a workflow to achieve full structural elucidation of trace impurities that can be implemented using preparative supercritical fluid chromatography to isolate trace impurities.

This application note demonstrates the successful chiral separations of fragrance compounds on ACQUITY UPC2 Trefoil AMY1 and CEL1 Columns using an ACQUITY UPC2 System. The low system volume and extra-column volume of the UPC2, combined with the reduced particle size of the ACQUITY UPC2 Trefoil AMY1 and CEL1 Columns, enable superior, faster, and more efficient separations compared with traditional SFC and GC.

Method development strategies in SFC often take a brute force screening approach utilizing extensive libraries of column chemistries with multiple combinations of modifiers and additives. This poster presents a simplified method development strategy due to the introduction of novel ACQUITY UPC²™ Torus™ 2-PIC, DIOL, DEA, and 1-AA column chemistries. Instead of using a multi-column / co-solvent initial screening, the simplified strategy implements a rapid scouting step using Torus 2-PIC with methanol co-solvent and generic gradient.

This application note will describe the rapid analysis of CBD from a several consumer products using UltraPerformance Convergence Chromatography™ (UPC2).

Watch this video to learn about fast, efficient method development using Torus and Trefoil column technology. To find out more, click on the webinar link below.

Discover the innovate technologies showcased at the Waters booth at Pittcon 2015