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Quantifying quality attributes of AAV gene therapy vectors by SEC-UV-MALS-dRI

3 Feb 2025

In this application note, Wyatt Technology present a simple and direct size-exclusion chromatography method with UV-MALS-dRI detection, which can be readily employed to quantify AAV particle concentration, capsid content, and aggregation throughout the AAV product development and manufacturing processes.

DAWN™

Waters | Wyatt Technology

The DAWN is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size, concentration and optional features.

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Quantifying quality attributes of AAV gene therapy vectors by SEC-UV-MALS-dRI