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PhyTip® Columns Containing CaptureSelect® Resins for Purification of Antibodies and Antibody Fragments

31 Jan 2013

In this application note the combination of PhyNexus separation columns and BAC resins is shown to provide an efficient and precise early stage evaluation for the spectrum of new developing antibody formats such as Fc fusions, Fab and FAb fragments, IgA’s, IgM’s, and IgG subclasses such as IgG4. Generating concentrated proteins from smaller volumes from more constructs earlier in the development process allows for additional analysis and subsequently better characterized biotherapeutics in the pipeline.

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PhyTip® Columns and MiniPrep PhyTip® Columns

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PhyTip® columns are the gold standard for high-throughput, small-scale purification of proteins, antibodies, plasmids, and viral vectors. By integrating advanced chromatography directly into a pipette tip, PhyTip® columns eliminate manual bottlenecks and deliver high quality purification with true "walk-away" automation.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.Antibody Purification

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