Multiscale Analysis of Bacteria Population in Legume Root Nodules with "Shuttle & Find"

4 May 2018

Correlative light and electron microscopy (CLEM) combines the overview information of fluorescence light microscopy (FLM) with structural details in scanning electron microscope (SEM) for high-content imaging. Hence, CLEM is very useful for investigating infection and colonization of the legume hosts by their rhizobial symbionts. This white paper illustrates a methodology involving the shuttle & find interface of CLEM to enable fast and reliable analysis of the bacterial infection of legume root nodule cells.

ZEISS Axio Imager - Modular System for Digital Fluorescence Microscopy

ZEISS Research Microscopy Solutions

Axio ImagerAn innovative modular system for digital fluorescence microscopy, featuring advanced flexibility and application versatility.  Featuring: New IC2S objectives (Infinity Contrast & Colour Corrected System) - optimise image quality and maximise contrast Special fluorescence filters - reduce exposure and image acquisition time for superior 3D imaging. 'Intelligent Stand' - automatically recognises added components.  "Contrast manager" ensures simple changes between contrasting techniques.  Integration: standard interfaces permit communication via USB and TCP/IP Vibration-free Imaging Cell - isolated within the stand for stable observation and unparalleled precision.  Apochromatic fluorescence beam path - ensures optimum colour correction Active stray light elimination - significantly higher contrast Fast, motorised reflector turrets - hold either six or ten filter modules.  HBO lamp: convenient, self-aligning homogeneous illumination LCI Plan-Neofluar objectives - live cell imaging Flexibility -  two freely configurable stand variants: Z1 (motorized) and D1(manual) or preconfigured stands M1 (motorized) and A1 (manual).  Rapid image acquisition with outstanding quality in up to 6 dimensions means more signal in less time.

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