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Exploring extracellular vesicles: Biomarkers and beyond

Biotium
15 Sept 2026

This application note explores the growing role of extracellular vesicles (EVs) in biomarker discovery, disease research, and therapeutic development, while highlighting challenges associated with EV detection and characterization. Learn how optimized fluorescent labeling strategies, EV-specific antibodies, and advanced flow cytometry approaches can improve signal-to-noise ratios and reduce false positives during EV analysis. Discover how Biotium’s ExoBrite™ stains and antibodies support more reliable EV detection, enabling accurate characterization of EV populations across a variety of research applications.

Resource details:

Resource type: Application note
Page count: 8
Read time: 12 mins

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ExoBrite™ True EV Membrane Stains

Biotium

The ExoBrite™ True EV Membrane Stains are designed to surpass the limitations of traditional membrane dyes and provide near-complete coverage of EVs with unmatched solubility, ensuring accurate and reliable results in your experiments.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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