Incubox reduces edge effects and improves consistency in long-term 3D assays

This application note demonstrates how the Incubox™ system from InSphero reduces evaporation-driven edge effects in long-term cell culture experiments. By maintaining a high-humidity microenvironment within standard incubators, the system stabilises media volume and compound concentration across all wells.

In a 14-day toxicity assay using liver microtissues, plates cultured in standard incubators showed significant variability and compromised viability in outer wells, while those maintained in the Incubox™ exhibited uniform performance across the entire plate. This enables full use of all wells and improves reproducibility in high-throughput workflows.

Resource details:

• Resource type: Application note
• Page count: 5
• Read time: 10 mins