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Improved Sensitivity of Hydrolyzed Urine Samples Using β-Gone™ β-Glucuronidase Removal Products
12 Feb 2017When the drugs exit the body through urine they are still in their glucuronide form and before chromatographic analysis the glucuronide bond must hydrolysed by β-glucuronidase, however residual β-glucuronidase and drug compounds can build up and negatively impact on the selectivity of the column. This application note describes a method for removing residual enzyme using β-Gone β-Glucuronidase Removal Products. This work demonstrates how these products can improve sensitivity while adding little variability to the results.
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Phenomenex Incβ-Gone β-Glucuronidase Removal 96-well plates and tubes are designed to target and remove β-glucuronidase from hydrolyzed urine samples without requiring additional time or method development. In a single step and in less than 1 minute, your hydrolyzed samples are ready for analysis. In Under a Minute You Can Easily: Increase HPLC/UHPLC Column Lifetime Reduce Mass Spec Maintenance Maintain the Selectivity of Your HPLC/UHPLC Column
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