Hydrophilic interaction liquid chromatography (HILIC) method migration part 1: From legacy HPLC systems to the Alliance iS HPLC System

In this application note, Waters helps illustrate a successful hydrophilic interaction liquid chromatography (HILIC) method migration from legacy HPLC systems found across industries to the Alliance iS HPLC System. Due to differences in HPLC instrument designs, HILIC method migration could be challenging since results could potentially be impacted. A well-designed system like Alliance iS HPLC System, helps overcome those challenges to allow for seamless migration. For this study, the United States Pharmacopeia (USP) monograph for cetirizine hydrochloride assay and organic impurities were analyzed on two legacy HPLC systems and migrated to the Alliance iS HPLC System. All three systems met suitability requirements and demonstrated comparable results.