How to isolate and count intact nuclei

Isolating clean, intact nuclei is vital for the success of single-nucleus workflows such as RNA sequencing (RNA-seq) and Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq). High-quality, debris-free samples are essential for accurate library preparation and downstream analyses, as debris can hinder precise nuclei counting. Traditional manual counting methods, while reliable, are time-consuming and prone to human error, especially with large sample volumes.

This eBook explores automated nuclei counting and quality control using the DeNovix CellDrop^™ Automated Cell Counter. With features like DirectPipette^™ technology that eliminates costly consumables, advanced fluorescence applications, and user-friendly software, researchers can improve accuracy, efficiency, and consistency in sample preparation. Discover practical tips to streamline workflows, minimize variability, and enhance experimental outcomes.