How to choose the optimum conditions for analysis of denatured or non-denatured siRNA duplexes

In this application note, YMC Europe introduces all relevant aspects of denaturing and non-denaturing ion pair reversed-phase high-performance liquid chromatography (IP-RP HPLC). As a sample, the small interfering RNA (siRNA) duplex targeting the firefly luciferase gene GL2 was used. siRNAs usually occur as oligonucleotide duplexes, they can be analyzed under non-denaturing conditions so that duplex formation is assured and excessive single-strands are detected. Alternatively, analysis of siRNAs can be conducted under denaturing conditions so that both sense and antisense strands are monitored.