Use case: His-tag protein expression screening from crude lysates
10 Nov 2025His-tagged proteins underpin work across structural biology, assay development, and drug discovery. When screening many constructs, teams need quick, quantitative readouts from crude lysates to decide what to scale and what to drop.
In this application note, Abselion demonstrates how by using a His-tag assay on the Amperia™ platform, teams can obtain reproducible concentration measurements directly from unpurified samples, informing expression and purification decisions earlier.
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Amperia™ Protein Quantification System
AbselionThe Amperia™ system combines compact hardware, assay-ready sensors, and integrated software to streamline protein quantification. Built to support scientists in discovery, development, and beyond, it delivers dependable data quickly and without unnecessary complexity — even from crude or complex samples.
Tagged Protein Quantification Kit | His-Tag (SKU: AK-HIS-004)
AbselionThe His-Tag Protein Quantification Kit extends the Amperia™ Protein Quantification System for direct, tag-specific measurement of His-tagged recombinant proteins. Using a premix competition format with GenScript’s THE™ His Tag Monoclonal Antibody, the kit delivers accurate, reproducible results across both crude lysates and purified samples, with up to 80 tests per kit.