Tagged Protein Quantification Kit | His-Tag (SKU: AK-HIS-004)
The His-Tag Protein Quantification Kit extends the Amperia™ Protein Quantification System for direct, tag-specific measurement of His-tagged recombinant proteins. Using a premix competition format with GenScript’s THE™ His Tag Monoclonal Antibody, the kit delivers accurate, reproducible results across both crude lysates and purified samples, with up to 80 tests per kit.
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Designed for scientists working in recombinant protein expression, purification, and screening, the His-Tag Protein Quantification Kit provides a ready-to-use solution for rapid, reliable quantification of His-tagged proteins.
Each kit includes pre-coated sensor strips, assay plates, and reagents for seamless integration with the Amperia™ system. The assay follows a premix competition format in which His-tagged proteins compete with HRP-labelled His-tagged detection reagents for anti-His capture sites on the sensor. The resulting inverse electrochemical signal allows quantification across a broad dynamic range, even in crude lysates.
Key Features:
- Ready-to-use format tailored for the Amperia™ system
- Tag-specific quantification using GenScript THE™ His Tag Monoclonal Antibody
- Premix competition format with inverse-signal detection
- Up to 80 tests per kit (5 sensor strips, 10 plates included)
- Compatible with crude lysates and purified samples
- Provides consistent, repeatable measurements.
Applications:
- Protein expression and purification monitoring
- Screening of His-tagged constructs or clones
- Optimisation of production conditions
- Comparative analysis of His-tagged variants
- Process development and yield assessment
Use case: His-tag protein expression screening from crude lysates
His-tagged proteins underpin work across structural biology, assay development, and drug discovery. When screening many constructs, teams need quick, quantitative readouts from crude lysates to decide what to scale and what to drop.
In this application note, Abselion demonstrates how by using a His-tag assay on the Amperia™ platform, teams can obtain reproducible concentration measurements directly from unpurified samples, informing expression and purification decisions earlier.
Enabling His-tagged protein analysis in expression workflows
His-tagging is a widely used strategy for protein purification, screening, and assay development – offering simple affinity-based capture without altering protein function.
In this application note, Abselion demonstrates Amperia™, offering a practical alternative, enabling quantification of His-tagged proteins even from crude samples using a premix competition assay.
Amperia: Your companion for fast, reliable, accessible protein quantification
In this product brochure, Abselion demonstrates the Amperia™, an at-line compact benchtop instrument, designed to be a simple-to-use and cost-effective solution for rapid and accurate automated quantification of a range of biomolecules directly from unpurified samples in standard 96-well microplates.
TechTalk: Fast, accurate, accessible quantification across diverse biologics
Friday, January 23 at 16:00 GMT | 17:00 CET | 11:00 EST | 08:00 PST
Quantifying antibodies, AAV capsids, and tagged proteins directly from crude or partially clarified samples remains a common bottleneck in many biologics workflows.
In this 20-minute TechTalk, Dr. Paolo Romele, VP of Science, Abselion will introduce the electrochemical approach used in the Amperia™ benchtop platform, which combines affinity-based capture with Redox Electrochemical Detection (RED) to provide quantitative measurements without relying on optical readouts.
Join this educational and technology-focused session to learn practical examples illustrating how these assays operate across biologics applications.
Key learning objectives:
- Understand the principles behind affinity-based electrochemical assays using Redox Electrochemical Detection (RED) as implemented in the Amperia™ system.
- Learn how the three assay formats are applied to antibodies, AAV capsids, and tagged proteins.
- Review example data demonstrating assay behaviour with directly prepared samples.
- See how electrochemical detection can complement existing analytical approaches in biologics measurement.
Who should attend?
- Biotech and pharma scientists working with antibodies, AAV vectors, tagged proteins, protein expression, cell line development, or vector optimization.
- Research teams in early development focused on process design, screening, assay development, and exploring quantification methods beyond traditional optical assays.
Certificate of attendance
If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.
Abselion launches His-tagged Protein Quantification Kit and Sensors for Amperia
Ready-to-use solution, developed in collaboration with GenScript, offers rapid and reliable quantification of His-tagged proteins
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