ResourceSeparations

High speed analysis of melamine in dairy

14 Mar 2022

In this application note, the analysis results of melamine in a milk product was measured using a PDA detector. Melamine is an organic nitrogen compound which has a triazine ring in the center of its structure. It is used as the material of melamine resin which has high thermal and water resistance and is used for mechanical strengthening.

LC-4000 Series PDA Detectors

JASCO (USA)

The LC-4000 Series includes three PDA detectors.The MD - 4 017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhor se for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution. The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detec tor. Making use of a 1024 element photometric diode array, the measurement wavelength range is from 190nm to 90 0nm with a MD-4010 PDA resolution of 1nm and fast spectrum acquisition rate of up to 100 spectra/ sec. When used with the new temperature controlled SP t ype semi-micro flow cell the MD-4010 is well suited to the narrower peaks typically found with UHPLC or RHPLC separations. The MD-4010 is fitted with an internal mercury lamp for automatic wavelength calibration. The MD-4015 PDA detector is the routine detector for conventional HPLC, RHPLC and UHPLC. The sensitivity is almost equivalent to that of the MD-4010 but only covers the wavelength range from 200nm to 600nm using a 512 element PDA for 1nm resolution and with high speed 100Hz spectrum acquisition.

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LC-4000 Series Integrated HPLC and UHPLC Systems

JASCO (USA)

The LC-4000 Series is the latest in a long history of innovative HPLC systems developed by JASCO reaching all the way back to the start of commercial HPLC in the early 1970s. The concept of the integrated LC-4000 series HPLC provides key separation platforms at 50MPa, 70MPa and 130MPa which correspond to conventional HPLC, the increasingly popular Rapid Analysis (RHPLC) and sub 2um UHPLC, respectively. Each platform is supplied with a dedicated pump and autosampler matched to the operating pressure and all three platforms share common detectors optimized for high-speed 100Hz acquisition and the narrow peak shapes common to both RHPLC and UHPLC. Features 300mm wide footprint Each system component is completely stackable into a modest height The system is fully integrated with complete system monitoring All user replaceable components are accessible through the front panel Vertical leak/drain system to channel away solvent Integrated earthquake securing option All detectors include 100Hz acquistion with interchangeable flowcells Preparative HPLC Systems The LC-4000 preparative HPLC systems includes three platform options Up to 20mL/min for columns up to 21.2mm ID Up to 50mL/min for columns up to 3cm ID Up to 120mL/min for columns up to 5cm ID

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High speed analysis of melamine in dairy