ResourceSeparations

Fatty acid methyl esters (FAMEs) analysis on an Agilent 8890 GC and its application to real samples

23 May 2021

This Application Note applies methods GB5009.168-20161 and 5009.257-20162 to FAMEs analysis using a long polar column in an Agilent 8890 GC. The GC method was optimized for the separation of 37 representative FAMEs and 21 representative trans FAMEs in 80 minutes. The system retention time (RT), area repeatability, and linearity were evaluated, and a FAMEs mixture prepared from real oil samples was analyzed.

J&W DB-FastFAME Columns

Agilent Technologies

Agilent J&W DB-FastFAME is a cyanopropyl phase engineered for the fast and selective separation of polysaturated and unsaturated FAMEs—even challenging cis and trans FAME isomers—without sacrificing the resolution generally achieved with traditional 100 meter long columns.

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Agilent 8890 GC System

Agilent Technologies

The 8890 Gas Chromatograph system meets specific analysis demands with speed, accuracy, and reliability. Built-in intelligence, 6th-generation electronic pneumatic control (EPC), and a unique browser interface that lets you monitor your system from home, office, or remote location increase productivity and uptime.

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Fatty acid methyl esters (FAMEs) analysis on an Agilent 8890 GC and its application to real samples