Direct measurements of cellular metabolism for identification of mitochondrial drug targets

In this application note, Agilent Technologies discusses the importance of drug target identification in drug discovery. Phenotypic screening is one method used, involving the addition of compounds to cells to observe their impact on cell activity. Agilent Seahorse XFe96 Analyzer is introduced as an ideal system for measuring mitochondrial respiration and cell metabolism in live cells, providing insights into metabolic drug targets. The workflow includes the Seahorse XF Cell Mito Stress Test to assess the compound's effect on mitochondrial function and subsequent XF assays with permeabilized cells to identify specific targets. Incorporating direct cell-based measurements offers valuable insights into compound effects and metabolic modulation for drug target identification.