Bringing oligonucleotide synthesis in-house

This resource provides practical guidance for molecular diagnostic laboratories, assay developers, and manufacturers evaluating whether to bring oligonucleotide synthesis in-house. It covers instrument selection, facility requirements, solvent handling, regulatory considerations, workflow planning, raw material sourcing, and oligo production processes using phosphoramidite chemistry.

The publication also compares the MerMade™ family of oligonucleotide synthesizers, ranging from low-throughput to high-throughput production systems, and discusses key synthesis steps including detritylation, coupling, capping, and oxidation.

The guide is intended to help laboratories improve supply chain control, accelerate turnaround times, protect intellectual property, and support assay development through internal oligo manufacturing capabilities.

Resource details:

Resource type: Application note
Page count: 18
Read time: 28 mins