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Analysis of pharmaceutical drugs with low mobile phase organic or aqueous percentages using the Agilent 1290 Infinity Quaternary LC System

6 Aug 2020

This Technical Note by Agilent provides two examples showing the performance of the 1290 Infinity Quaternary Pump below 5% and above 90% organic phase.

Agilent 1290 Infinity III Flexible Pump

Agilent Technologies

The 1290 Infinity III Flexible Pump is a quaternary pump that provides UHPLC gradient formation and solvent blending from up to four solvents. This pump enables quaternary solvent delivery at pressures up to 1300 bar and flow rates up to 5 mL/min.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Analysis of pharmaceutical drugs with low mobile phase organic or aqueous percentages using the Agilent 1290 Infinity Quaternary LC System