Advances in Size Exclusion Chromatography for the Analysis of Macromolecular Proteins

Size exclusion chromatography has been the preferred method for the analysis of proteins based on size. This application note demonstrates how by combining 450 Å sub 3-µm packing materials with a low dispersion ACQUITY UPLC H-Class System, separations with improved resolution and high-throughput of SE-UPLC can be realized for macromolecular proteins and highly aggregated proteins with molecular weights of up to approximately 2000 kDa.