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A Standardized, Automated Approach for Exosome Isolation and Characterization Using Beckman Coulter Instrumentation

Beckman Coulter Diagnostics
7 Jun 2016

This application note describes a workflow using automated Biomek methods for centrifugation layering and fractionation, total RNA extraction, and cDNA amplification and clean-up for next generation sequencing. NGS results are reported on benign and cancerous colon cell lines.

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Tags

Cell / Tissue CultureGenome AnalysisCell Lines Stem Cells and Primary CellsCentrifugesSample ManagementCell-Based AssaysDNA / RNA Extraction and PurificationDNA SequencingNanotechnologyGene Expression and Molecular CloningRNA InterferenceBiomarkersRobotic InstrumentsSoftware PlatformsClinical Genetics

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