Cell-Based Assays > Protein Labeling Kits Products & Reviews

based on the dye-binding properties of Coomassie Blue G-250

Imaging probes conjugated to IRDye 800CW and IRDye 700DX have propelled more than 20 Phase I and Phase II clinical trials worldwide.

EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.

The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemilu…

Precision-engineered cell mimics with integrated fluorescent proteins

The Quick Western Kit provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step.

IRDye 680RD is the near-infrared fluorescent dye of choice for applications in the 700 nm wavelength, especially Western blots, cell-based assays such as the In-Cell Western assay, and small animal imaging experiments.

IRDye QC-1 is the first non-fluorescent (dark) quencher, compatible with a wide range of visible and near-infrared fluorophores (~500 – 800 nm).

IRDye® Click Chemistry reagents (DBCO, Alkyne, and Azide) offer convenient, versatile and reliable methods for biomolecule labeling. With a variety of in vitro and in vivo applications, from biomarker isolation to assay development.

IRDye Labeling Kits come in 3 forms and allow you to label your own peptides, antibodies, or proteins.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the wel…

This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.

This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a s…

GFP/RFP-Booster – enhance your fluorescent fusion protein ChromoTek’s Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC. With our Booster you can: • Reactivate • Boost • Stabilize the signals of your fusion proteinsDirectly stain your protein of interest in fixed cells and benefit from the high specificity an small size that are perfect for su…

Chromobodies® - Your perfect reagents for cellular research including High-Content Analysis. Chromobodies are fluorescent nanoprobes suitable for real time analyses of endogenous cellular structures and processes in live cells. Chromobodies® are a novel species of extremely small intracellular antibodies. They are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a f…

DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…

Rapid, easy to use protein labeling kit from Fluidic Analytics.

Biotinylated Hyaluronan Binding protein (b-HABP) (versican G1 domain, VG1)

Label your antibody with one of our superior CF ®  Dyes in 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF has been proven in a variety of applications including GPCRs, kinases, epigenetics, PPIs, and the quantification of a wide range of biomarkers including cytokines.

CDP-Star® and CSPD® chemiluminescent substrates for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.

Chemiluminescent substrate concentrates for E.coli ß-galactosidase assays.