Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Label your antibody with one of our superior CF ® Dyes in 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.
Overview of workflow.
Labeling kit comparison
Place your order directly with the manufacturer.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 15 minutes, with minimal hands-on time and no purification.
Features
- Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Choice of 32 CF® Dye colors
- Less than 30 seconds of hands-on time
- 15 minutes total reaction time
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive CF® Dye
- Mix-n-Stain™ Storage Buffer
Unrivaled Convenience
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.
Superior CF® Dyes
These kits feature a choice of 32 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.
Other Labeling Kits
Biotium also offers Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG.
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.
CF Dyes & cell stains for microscopy
This scientific poster serves as a comprehensive reference for fluorescence microscopy, showcasing excitation and emission spectra of over 40 fluorophores, including CF® Dyes and common cellular stains. It also features laser excitations and emission filter sets for typical microscope configurations, making it a valuable tool for designing experiments. Ideal for lab display, it provides quick access to key fluorescence imaging data.
Fluorophore selection guide for microscopy
Explore excitation and emission spectra of Biotium's bright and photostable CF® Dyes along with other popular cellular stains and dyes for fluorescence microscopy.
CF Dyes: Clearer fluorescent results
Fluorescent dyes are used by researchers for numerous applications such as labeling cells to study their behaviors, quantifying and characterizing cell activity during systemic responses, investigating protein-protein interactions, tracking signaling pathways, and understanding tissue structure and composition. In this eBook, Biotium covers the history of fluorescent dyes in life science research, and provides an overview of fluorescent dye technology, highlighting the advantages of CF® Dyes and their ability to probe deeper into biological samples. Additionally, Biotium provides an infographic that illustrates how CF Dyes can illuminate pathways to discovery in various research applications such as super-resolution microscopy or spectral flow cytometry.
How better probes give clearer fluorescent results
In this video, Biotium outlines how its scientists have developed fluorescent labels that promise to offer the best possible performance for life science research. CF® Dyes provide clearer fluorescent results for western blotting, microscopy, flow cytometry, and other applications. The CF Dyes are available as primary and secondary antibodies, conjugates, reactive dyes, and labeling kits and are ideal for near-IR western or small animal imaging.
