Enzymes Products & Reviews

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

UltraScript® 2.0 cDNA Synthesis Kit is a 2-tube kit containing all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types.

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. 

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.

VeriFi® Hot Start Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

PCRBIO HiFi Polymerase is our original high-fidelity enzyme, derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates, with a red dye included for direct gel loading.

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The advanced buffer system, reverse transcriptase and hot start polymerase give highly specific and ultra-sensitive 1-step RT-PCR from any RNA template including mRNA, total RNA and viral RNA sequences.

IsoFast® Bst Polymerase is a recombinant form of the large fragment of Bst DNA polymerase, containing 5′-3′ polymerase activity and strand displacement activity.

IsoFast® Bst 1-Step Mix is a dual enzyme system for rapid and sensitive isothermal amplification of RNA targets in one step.

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

IsoFast® Hot Start Bst Polymerase is the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes. This mix is suitable for applications such as multiple displacement amplification, isothermal amplification, loop mediated isothermal amplification (LAMP), molecular diagnostics and field diagnostics.

IsoFast® Hot Start Bst Mix is the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes. This mix is suitable for applications such as multiple displacement amplification, isothermal amplification, loop mediated isothermal amplification (LAMP), molecular diagnostics and field diagnostics.

IsoFast® Hot Start Bst Colour Mix is a colourimetric isothermal amplification enzyme formulation that combines IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid positive/negative screening.

resDetect™ DNase Activity Assay Kit (Fluorescence) uses the FRET method to detect DNase in buffers, reagents, and other components within 30 minutes. It offers a rapid, sensitive, and quantitative tool for identifying DNase contamination. Additionally, the DNase and RNase Activity Assay Kit enables simultaneous quantitative detection of DNases and RNases in a single assay.

resDetect™ RNase Activity Assay Kit (Fluorescence) uses the FRET method to detect RNase in buffers, reagents, and other components within 30 minutes. It offers a rapid, sensitive, and quantitative tool for identifying RNase contamination. Additionally, the DNase and RNase Activity Assay Kit enables simultaneous quantitative detection of DNases and RNases in a single sample.

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…

IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus. It is an engineered recombinant protease overexpressed in E. coli. Like IdeS Protease, IdeZ Protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations